tinto rang™ is the safest and fastest fluorescent dye outperforms ethidium bromide in any gel system, including agarose and polyacrylamide gels. It is the most sensitive stain for dsDNA, ssDNA and RNA. tinto rang™ can be applied in many fields of study including clinical research and diagnostics, academic research, agricultural research, food safety testing, environmental monitoring, and forensic analysis.
tinto rang™ is a minor groove / external binding nucleic acid dye that has high affinity and rapidly binds to DNA. It may find potential applications in the making of safe, fluorescence-based Minor Groove Binder (MGB) dyes and probes that have several advantages over DNA intercalating probes in terms of increased stability, sensitivity and specificity.
However, there are various problems associated with SYBR Green-I, such as:
Because of these issues, there is an increased demand for Minor Groove Binding (MGB) dyes and probes that would not interfere with the amplification process.
EtBr vs tinto rang™ in DNA Forensics
|Methods||Ethidium Bromide||tinto rang™|
|In-gel staining||Not recommended; affects mobility of DNA and thereby, the pattern||Does not affect mobility and DNA pattern|
|Re-extraction of samples||Due to its intercalating property, alters DNA structure that may affect results||MGB/EB property does not alter DNA structure and thereby allows reuse of limited DNA samples|
|Handling and disposal concerns after post staining||Potent mutagen and carcinogen||Food grade; No disposal issues|
|Post staining time||10- 20 minutes||1- 10 minutes*|
* Depends on the sample and its concentration.