Detection & Visualization
tinto rang™ is a minor groove / external binding nucleic acid dye that has a high affinity and rapid binding to DNA. It may find potential applications in the making of safe, fluorescence-based Minor Groove Binder (MGB) dyes and probes that have several advantages over DNA intercalating probes in terms of increased stability, sensitivity and specificity.
Quantification and analysis of quality of the DNA are necessary to ascertain the approximate quantity of DNA obtained and the suitability of DNA sample for further analysis. This is important for many applications including digestion of DNA by restriction enzymes or PCR amplification of target DNA, gene expression analysis, genotyping and others.
Molecular diagnostics is a collection of techniques used to analyse biological markers in the genome and proteome. Molecular diagnostic tests detect specific sequences in DNA or RNA that may or may not be associated with disease, including single nucleotide polymorphism (SNP), deletions, rearrangements, insertions and others. Clinical applications can be found in at least six general areas: infectious diseases; oncology; pharmacogenomics; genetic disease screening; human leukocyte antigen typing; and coagulation.
Each molecular assay requires three basic steps:The extraction and purification of nucleic acid.
The amplification or making copies of the nucleic acid of interest (target) or attaching multiple copies of a dye to a single target copy.
The detection of the amplified target using real time polymerase chain reaction (PCR) or end product detection including microarrays, Luminex (similar to flow cytometry), or sequencing.