tinto rang™ DNA gel stain is compatible with all downstream applications we have tested so far, including existing PCR products from gels, gel purification, Gateway® cloning, TOPO® cloning, and restriction enzyme cloning. If you have a unique application that works with tinto rang™ DNA gel stain, send us the details at firstname.lastname@example.org
It is sufficiently stable to withstand UV illumination for >30 minutes; realistically, hours of constant UV or bright room light exposure are required to cause any significant loss of signal. We recommend that tinto rang™ DNA gel stain be protected from light during storage.
We have found a distinct advantage to using tinto rang™ DNA gel stain and non-UV blue light rather than ethidium bromide and UV when purifying DNA from gels for downstream use. The use of tinto rang™ DNA gel stain with non-UV blue light emitted by the E-Gel® Imager System with Blue Light Base allows you to purify DNA with virtually no UV-induced nicking or crosslinking, resulting in dramatically increased cloning efficiencies.
Similar to ethidium bromide, tinto rang™ DNA gel stain runs in the opposite direction from that of the migrating DNA. This has no practical effect on the use of gels cast with tinto rang™ DNA gel stain, as only the very bottom of the gel will have a lower concentration of stain. How much of the gel will end up with a lower stain concentration is highly dependent on the agarose concentration, buffer used, and electrophoresis conditions (voltage, wattage, length of run, etc).This effect can be partially counteracted by adding tinto rang™ DNA gel stain to the running buffer.
Double-stranded DNA-binding dyes are dyes that bind to the minor groove of double-stranded DNA (dsDNA). The free dye has a very low fluorescence, whereas the bound dye has a high fluorescence. The more double-stranded PCR product that is produced, the greater the fluorescence signal will be. tinto rang™ Dye is an example of a dsDNA-binding dye. These dyes allow you to use standard PCR primers, so there is no need to order special labeled primers.
tinto rang™ DNA gel stain has two main excitation peaks: in the UV region at 280 nm, and in the visible region at 502 nm. Thus, 254 nm or 300 nm UV excitation will work, as will 488 nm lasers, 470 nm LEDs, and broad blue excitation (such as the Safe Imager™ blue-light transilluminator (Cat. No. G6600). Maximal excitation occurs at 502 nm; the Safe Imager™ blue-light transilluminator is therefore the best choice for excitation of tinto rang™ DNA gel stain. The full excitation and emission spectra for tinto rang™ DNA gel stain are provided online and can also be found in the protocol provided with the stain.