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Tedious Time Consuming Protocols

“ EtBr Waste must be collected & managed as Hazardous Waste”

Various Bio-Hazardous Nucleic Acid Stains

Nucleic Acid Stains Mutagenic Toxicity Sensitivity*(ng) Binding Mechanism Cell Permeable** Excitation (nm) Emission (nm) Safe to Handle
Ethidium Bromide Strong Mutagenic Yes 2.5 Intercalator Yes 210, 285 605 No
SYBR Safe Weak Mutagenic No 1-5 Intercalator Yes 410-510 560/50 No
SYBR Green I Weak Mutagenic Yes 0.5 Intercalator Yes 494 520 No
SYBR Green II Weak Mutagenic Yes 0.5 Intercalator Yes 492 513 No
SYBR Gold Weak Mutagenic No 0.025 Intercalator Yes 300 495/537 No
GelRed Weak Mutagenic No 0.5 Intercalator NO 300, 250 600 No
GelGreen Weak Mutagenic No 0.5 Intercalator NO 495 520 No
Diamond Weak Mutagenic Only at stock concentration (10 000X) 0.5 External Binder Yes 494 558 No
Midori Green Direct Weak Mutagenic No 0.8 Non-intercalating(In-Gell Staining) Yes 290,500 530 No
Midori Green Advance Weak Mutagenic No 0.8 Non-intercalating(In-Gell Staining) Yes 270,290,490 530 No
EvaGreen Weak Mutagenic Yes 0.5 Intercalator Yes 488,500 530 No
RedSafe Less Mutagenic Yes 5 Unknown Yes 309, 419, 514 537 No